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Chemical and Functional Genomic Approaches to Stem Cell Biology and Regenerative Medicine

2008-02-08 Medical
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Release : 2008-02-08
Genre : Medical
Kind : eBook
Book Rating : 121/5 ( reviews)

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Book Synopsis Chemical and Functional Genomic Approaches to Stem Cell Biology and Regenerative Medicine by : Sheng Ding

Download or read book Chemical and Functional Genomic Approaches to Stem Cell Biology and Regenerative Medicine written by Sheng Ding. This book was released on 2008-02-08. Available in PDF, EPUB and Kindle. Book excerpt: Scientists believe that stem cells have the potential to revolutionize the treatment of numerous diseases and conditions. This guide covers recent advances in technologies and their applications in stem cell biology, addressing the use of both embryonic and adult stem cells and discussing diverse technologies, including genome-wide expression analysis, informatics, chemical genomics, and more. Applications covered encompass self-renewal, differentiation, reprogramming, and regeneration in model organisms. This is a premier reference for practicing professionals involved in stem cell research and students.

Functional Genomic Approaches Toward the Regenerative Medicine

2003 Chemistry, Organic
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Release : 2003
Genre : Chemistry, Organic
Kind : eBook
Book Rating : /5 ( reviews)

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Book Synopsis Functional Genomic Approaches Toward the Regenerative Medicine by : Sheng Ding

Download or read book Functional Genomic Approaches Toward the Regenerative Medicine written by Sheng Ding. This book was released on 2003. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in stem cell and developmental biology have great implications for treating many devastating diseases, such as Parkinson's disease, diabetes, liver failure, and cancer via cell-based therapy and/or by stimulation of tissue regeneration in vivo . However, a number of obstacles must be overcome before we can fully harness their immense therapeutic potentials in mammalian regeneration. This requires a better understanding of the signaling pathways that control cell fate and an improved ability to manipulate stem cell proliferation and differentiation. Therefore we have developed various functional genomic technologies (including combinatorial technologies, genome-wide gene knock-in and knock-out technologies using arrayed cDNA and siRNA libraries, and high throughput phenotypic cell-based screens) to identify small molecules and genes that can permit precise regulation of stem cell fate in a wide variety of systems including pluripotent embryonic stem cells and adult neural stem cells.

Three Functional Genomic Approaches to Biochemical and Screen-based Analyses of Topics in Cellular Biology

2007 DNA microarrays
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Release : 2007
Genre : DNA microarrays
Kind : eBook
Book Rating : 907/5 ( reviews)

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Book Synopsis Three Functional Genomic Approaches to Biochemical and Screen-based Analyses of Topics in Cellular Biology by : ́ Jovana J. Grbic

Download or read book Three Functional Genomic Approaches to Biochemical and Screen-based Analyses of Topics in Cellular Biology written by ́ Jovana J. Grbic. This book was released on 2007. Available in PDF, EPUB and Kindle. Book excerpt: The central dogma of molecular biology states that cellular regulation on a most basic level is carried out by the process of DNA transcription to mRNA, which is then translated to protein. In addition to representing key pieces of cellular machinery responsible for genetic function in higher organisms, one may also view these as dynamic and malleable tools for evaluating disease and biological models on a genome-wide approach. Presented herein is a triumvirate of projects that bridge the interface of functional genomics on a DNA, RNA and protein level and basic biology. Chapter 1 discusses the DNA profiling of the Runx3 tumor suppressor employing a gastric cancer cellular model system. Expression analysis of downregulated genes in the presence of reconstituted Runx3 provided key insights into the molecular pathways silenced by this gene. Chapter 2 details the design and high-throughput construction of a novel shDNA library (vector-based RNAi) targeting the murine kinome and its use in a loss-of-function screen to identify putative kinase regulators of osteocyte formation. Lastly, Chapter 3 illustrates a successful application of protein-based immunoprecipitation/mass spectroscopy (IPMS) to identify a novel binding partner, Pontin52, of the hematopoietic stem cell regulator and oncogene Bmi1. Gene-specific knockdown of both targets incurred cancer-cell specific apoptosis. In all three cases, a broad initial approach allowed us to hone in on key genes of interest that would otherwise have been difficult to pinpoint, which facilitated further characterizations of their respective biological roles in the three systems described.

Next Steps for Functional Genomics

2020-12-18 Science
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Release : 2020-12-18
Genre : Science
Kind : eBook
Book Rating : 738/5 ( reviews)

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Book Synopsis Next Steps for Functional Genomics by : National Academies of Sciences, Engineering, and Medicine

Download or read book Next Steps for Functional Genomics written by National Academies of Sciences, Engineering, and Medicine. This book was released on 2020-12-18. Available in PDF, EPUB and Kindle. Book excerpt: One of the holy grails in biology is the ability to predict functional characteristics from an organism's genetic sequence. Despite decades of research since the first sequencing of an organism in 1995, scientists still do not understand exactly how the information in genes is converted into an organism's phenotype, its physical characteristics. Functional genomics attempts to make use of the vast wealth of data from "-omics" screens and projects to describe gene and protein functions and interactions. A February 2020 workshop was held to determine research needs to advance the field of functional genomics over the next 10-20 years. Speakers and participants discussed goals, strategies, and technical needs to allow functional genomics to contribute to the advancement of basic knowledge and its applications that would benefit society. This publication summarizes the presentations and discussions from the workshop.

Functional Genomics Approaches for Understanding Neurological Disorders

2015
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Release : 2015
Genre :
Kind : eBook
Book Rating : 458/5 ( reviews)

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Book Synopsis Functional Genomics Approaches for Understanding Neurological Disorders by : Matthew Anthony Lalli

Download or read book Functional Genomics Approaches for Understanding Neurological Disorders written by Matthew Anthony Lalli. This book was released on 2015. Available in PDF, EPUB and Kindle. Book excerpt: The commencement of my doctoral studies coincided with the emergence of two groundbreaking scientific achievements: whole-genome sequencing and induced pluripotent stem cells. Here, I used these seminal technological advancements in biology as novel approaches to establish phenotype-genotype connections in neurological disorders. Functional genomics aims to integrate high throughput genomic and transcriptomic data to understand the biological function of genetic variation. This dissertation is split into two parts presenting divergent functional genomics approaches to understand two neurological disorders: whole-genome sequencing in Alzheimer's disease and induced pluripotent stem cell modeling of Williams syndrome.

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