Author : ́ Jovana J. Grbic
Release : 2007
Genre : DNA microarrays
Kind : eBook
Book Rating : 907/5 ( reviews)
Book Synopsis Three Functional Genomic Approaches to Biochemical and Screen-based Analyses of Topics in Cellular Biology by : ́ Jovana J. Grbic
Download or read book Three Functional Genomic Approaches to Biochemical and Screen-based Analyses of Topics in Cellular Biology written by ́ Jovana J. Grbic. This book was released on 2007. Available in PDF, EPUB and Kindle. Book excerpt: The central dogma of molecular biology states that cellular regulation on a most basic level is carried out by the process of DNA transcription to mRNA, which is then translated to protein. In addition to representing key pieces of cellular machinery responsible for genetic function in higher organisms, one may also view these as dynamic and malleable tools for evaluating disease and biological models on a genome-wide approach. Presented herein is a triumvirate of projects that bridge the interface of functional genomics on a DNA, RNA and protein level and basic biology. Chapter 1 discusses the DNA profiling of the Runx3 tumor suppressor employing a gastric cancer cellular model system. Expression analysis of downregulated genes in the presence of reconstituted Runx3 provided key insights into the molecular pathways silenced by this gene. Chapter 2 details the design and high-throughput construction of a novel shDNA library (vector-based RNAi) targeting the murine kinome and its use in a loss-of-function screen to identify putative kinase regulators of osteocyte formation. Lastly, Chapter 3 illustrates a successful application of protein-based immunoprecipitation/mass spectroscopy (IPMS) to identify a novel binding partner, Pontin52, of the hematopoietic stem cell regulator and oncogene Bmi1. Gene-specific knockdown of both targets incurred cancer-cell specific apoptosis. In all three cases, a broad initial approach allowed us to hone in on key genes of interest that would otherwise have been difficult to pinpoint, which facilitated further characterizations of their respective biological roles in the three systems described.