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Advanced Time-Correlated Single Photon Counting Techniques

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Release : 2005-12-19
Genre : Science
Kind : eBook
Book Rating : 821/5 ( reviews)

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Book Synopsis Advanced Time-Correlated Single Photon Counting Techniques by : Wolfgang Becker

Download or read book Advanced Time-Correlated Single Photon Counting Techniques written by Wolfgang Becker. This book was released on 2005-12-19. Available in PDF, EPUB and Kindle. Book excerpt: In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

Time-correlated single photon counting

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Release : 2012-12-02
Genre : Science
Kind : eBook
Book Rating : 447/5 ( reviews)

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Book Synopsis Time-correlated single photon counting by : Desmond O'Connor

Download or read book Time-correlated single photon counting written by Desmond O'Connor. This book was released on 2012-12-02. Available in PDF, EPUB and Kindle. Book excerpt: Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Advanced Photon Counting

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Release : 2015-04-23
Genre : Science
Kind : eBook
Book Rating : 365/5 ( reviews)

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Book Synopsis Advanced Photon Counting by : Peter Kapusta

Download or read book Advanced Photon Counting written by Peter Kapusta. This book was released on 2015-04-23. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Photon-Counting Image Sensors

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Release : 2018-07-06
Genre : Technology & Engineering
Kind : eBook
Book Rating : 742/5 ( reviews)

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Book Synopsis Photon-Counting Image Sensors by : Eric R. Fossum

Download or read book Photon-Counting Image Sensors written by Eric R. Fossum. This book was released on 2018-07-06. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Photon-Counting Image Sensors" that was published in Sensors

Photon Counting

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Release : 2018-03-21
Genre : Science
Kind : eBook
Book Rating : 07X/5 ( reviews)

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Book Synopsis Photon Counting by : Nikolay Britun

Download or read book Photon Counting written by Nikolay Britun. This book was released on 2018-03-21. Available in PDF, EPUB and Kindle. Book excerpt: Photon counting is a unified name for the techniques using single-photon detection for accumulative measurements of the light flux, normally occurring under extremely low-light conditions. Nowadays, this approach can be applied to the wide variety of the radiation wavelengths, starting from X-ray and deep ultraviolet transitions and ending with far-infrared part of the spectrum. As a special tribute to the photon counting, the studies of cosmic microwave background radiation in astronomy, the experiments with muon detection, and the large-scale fundamental experiments on the nature of matter should be noted. The book provides readers with an overview on the fundamentals and state-of-the-art applications of photon counting technique in the applied science and everyday life.

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