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Advanced Time-Correlated Single Photon Counting Techniques

2005-12-19 Science
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Release : 2005-12-19
Genre : Science
Kind : eBook
Book Rating : 821/5 ( reviews)

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Book Synopsis Advanced Time-Correlated Single Photon Counting Techniques by : Wolfgang Becker

Download or read book Advanced Time-Correlated Single Photon Counting Techniques written by Wolfgang Becker. This book was released on 2005-12-19. Available in PDF, EPUB and Kindle. Book excerpt: In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

Time-correlated single photon counting

2012-12-02 Science
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Release : 2012-12-02
Genre : Science
Kind : eBook
Book Rating : 447/5 ( reviews)

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Book Synopsis Time-correlated single photon counting by : Desmond O'Connor

Download or read book Time-correlated single photon counting written by Desmond O'Connor. This book was released on 2012-12-02. Available in PDF, EPUB and Kindle. Book excerpt: Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Advanced Photon Counting

2015-04-23 Science
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Release : 2015-04-23
Genre : Science
Kind : eBook
Book Rating : 365/5 ( reviews)

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Book Synopsis Advanced Photon Counting by : Peter Kapusta

Download or read book Advanced Photon Counting written by Peter Kapusta. This book was released on 2015-04-23. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Advanced Time-Correlated Single Photon Counting Applications

2015-04-13 Science
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Release : 2015-04-13
Genre : Science
Kind : eBook
Book Rating : 296/5 ( reviews)

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Book Synopsis Advanced Time-Correlated Single Photon Counting Applications by : Wolfgang Becker

Download or read book Advanced Time-Correlated Single Photon Counting Applications written by Wolfgang Becker. This book was released on 2015-04-13. Available in PDF, EPUB and Kindle. Book excerpt: This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Advanced Photon Counting

2015
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Release : 2015
Genre :
Kind : eBook
Book Rating : 378/5 ( reviews)

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Book Synopsis Advanced Photon Counting by : Peter Kapusta

Download or read book Advanced Photon Counting written by Peter Kapusta. This book was released on 2015. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

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