1# Library to Find Best eBook for Read
Author : Jan Neumann
Release : 2018
Genre :
Kind : eBook
Book Rating : /5 ( reviews)
Book Synopsis Development and Application of Advanced Fluorescence Microscopy Techniques for the Investigation of Inflammatory Processes by : Jan Neumann
Download or read book Development and Application of Advanced Fluorescence Microscopy Techniques for the Investigation of Inflammatory Processes written by Jan Neumann. This book was released on 2018. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Natalia Grigoryeva
Release : 2020-09
Genre : Cytofluorometry
Kind : eBook
Book Rating : 393/5 ( reviews)
Book Synopsis Fluorescence Methods for Investigation of Living Cells and Microorganisms by : Natalia Grigoryeva
Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva. This book was released on 2020-09. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Author :
Release : 2023
Genre :
Kind : eBook
Book Rating : 207/5 ( reviews)
Book Synopsis Development of Advanced Single-molecule Fluorescence Microscopy Techniques and Their Application for the Study of Protein-protein Interactions in the Context of Immunological Signaling by :
Download or read book Development of Advanced Single-molecule Fluorescence Microscopy Techniques and Their Application for the Study of Protein-protein Interactions in the Context of Immunological Signaling written by . This book was released on 2023. Available in PDF, EPUB and Kindle. Book excerpt: This thesis titled ``Development of Advanced Single-Molecule Fluorescence Microscopy Techniques and their Application for the Study of Protein-Protein Interactions in the Context of Immunological Signaling'' aims to make single-molecule imaging and the study of protein-protein interactions on membranes more accessible to the research community by providing new single-molecule fluorescence microscopy tools and techniques, and sharing them following an open-science approach. Besides the design, assembly, and characterization of a custom single-molecule TIRF microscope specifically tailored for live-cell and single-molecule imaging applications, the author presents an experimental platform designed for single-molecule analysis of ligand-induced binding, which was employed to investigate purified interleukin-2 and -15 receptors reconstituted on supported lipid bilayers. Furthermore, the author presents a novel approach called DNA-PAINT single-particle tracking (DNA-PAINT-SPT), an innovative method designed to extend single-molecule trajectory lengths of membrane proteins in live cells and reconstitution experiments. In the thesis, this method is applied for extensive quantitative studies of the binding kinetics of a membrane-anchored protein homodimer. Overall, the developments and applications presented in this thesis contribute to the advancement of single-molecule fluorescence microscopy techniques and open up new possibilities for investigating protein-protein interactions and immunological signaling in greater detail.
Author : X. F. Wang
Release : 1996-04-25
Genre : Science
Kind : eBook
Book Rating : 277/5 ( reviews)
Book Synopsis Fluorescence Imaging Spectroscopy and Microscopy by : X. F. Wang
Download or read book Fluorescence Imaging Spectroscopy and Microscopy written by X. F. Wang. This book was released on 1996-04-25. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the introduction of the combined use of fluorescence spectroscopy and microscopy has opened up exciting new arenas of scientific investigation. The combination of spectroscopic techniques and video microscopy enables acquisition of quantitative data about dynamic processes. It allows the investigation of molecular activity as it occurs--at the level of a single, intact, living cell--and provides extraordinary detail regarding biological organization and function. This new work covers emerging technologies and their applications in a variety of disciplines, including chemistry, biology, physics, computer science, engineering, medicine, and pharmacology. These topics are presented by contributing authors who are leading--and pioneering--researchers in their fields. They offer a critical evaluation of both theoretical and practical aspects of the use of these combined technologies in a way that cuts across disciplines, promoting interactivity and dialogue among scientists in different fields. Each chapter is devoted to a new, unique, or otherwise novel application of the combination of imaging techniques and fluorescence spectroscopy. In addition to covering established methods and instrumentation, the contributors also identify potential new applications in a number of areas. Throughout, they emphasize the multifunctional and multidimensional uses of the marriage of fluorescence imaging spectroscopy and digital imaging microscopy. They show how these technologies can be used in combination to obtain distinct yet complementary information, allowing two- and three-dimensional measurements, as well as manipulations of the sample under study in real time. This book makes the field of fluorescence imaging spectroscopy and microscopy accessible to scientists in a variety of research fields. It is also an excellent text for graduate students, a reference to the latest techniques, and a source of ideas to stimulate future investigation. An exploration of ideas, methods, and applications in the exciting new area of fluorescence imaging spectroscopy and microscopy This book covers the latest in techniques and advances that have developed from the combined use of fluorescence imaging spectroscopy and video microscopy. A relatively new innovation, it has revolutionized molecular research in a wide range of disciplines. This approach allows acquisition of quantitative data regarding real-world biological processes--providing unprecedented insight into the physiology of living cells and their molecular structures and functions. Written by a diverse group of leading researchers who contribute from their respective scientific disciplines--and from their perspective at the cutting edge of the technology--this book * Combines a unified treatment and a critical evaluation of the field, presenting new ideas, methods, and instrumentation, with tips for developing new applications * Covers many new techniques in different scientific disciplines--from biology and chemistry to physics, engineering, and medicine * Emphasizes the multifunctional and the multidimensional uses of fluorescence spectroscopy and video microscopy, and shows how they can be applied in different combinations to obtain new information For biochemists, cell biologists, biophysicists, physiologists, neuroscientists, applied physicists, and materials scientists, as well as for scientists working in industrial, agricultural, medical, and pharmaceutical research--and for graduate students in these areas--this book is a novel resource of research opportunities, new ideas, and current practices in a dynamic and burgeoning new field.
Author : Partha Pratim Mondal
Release : 2016-08-23
Genre : Science
Kind : eBook
Book Rating : 661/5 ( reviews)
Book Synopsis Fundamentals of Fluorescence Microscopy by : Partha Pratim Mondal
Download or read book Fundamentals of Fluorescence Microscopy written by Partha Pratim Mondal. This book was released on 2016-08-23. Available in PDF, EPUB and Kindle. Book excerpt: This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
