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Handbook of Fluorescence Spectroscopy and Imaging

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Release : 2010-12-23
Genre : Science
Kind : eBook
Book Rating : 529/5 ( reviews)

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Book Synopsis Handbook of Fluorescence Spectroscopy and Imaging by : Markus Sauer

Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer. This book was released on 2010-12-23. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Fluorescence Spectroscopy and Microscopy

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Author :
Release : 2014
Genre : Fluorescence microscopy
Kind : eBook
Book Rating : 498/5 ( reviews)

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Book Synopsis Fluorescence Spectroscopy and Microscopy by : Yves Engelborghs

Download or read book Fluorescence Spectroscopy and Microscopy written by Yves Engelborghs. This book was released on 2014. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

Fluorescence Imaging Spectroscopy and Microscopy

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Author :
Release : 1996-04-25
Genre : Science
Kind : eBook
Book Rating : 277/5 ( reviews)

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Book Synopsis Fluorescence Imaging Spectroscopy and Microscopy by : X. F. Wang

Download or read book Fluorescence Imaging Spectroscopy and Microscopy written by X. F. Wang. This book was released on 1996-04-25. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the introduction of the combined use of fluorescence spectroscopy and microscopy has opened up exciting new arenas of scientific investigation. The combination of spectroscopic techniques and video microscopy enables acquisition of quantitative data about dynamic processes. It allows the investigation of molecular activity as it occurs--at the level of a single, intact, living cell--and provides extraordinary detail regarding biological organization and function. This new work covers emerging technologies and their applications in a variety of disciplines, including chemistry, biology, physics, computer science, engineering, medicine, and pharmacology. These topics are presented by contributing authors who are leading--and pioneering--researchers in their fields. They offer a critical evaluation of both theoretical and practical aspects of the use of these combined technologies in a way that cuts across disciplines, promoting interactivity and dialogue among scientists in different fields. Each chapter is devoted to a new, unique, or otherwise novel application of the combination of imaging techniques and fluorescence spectroscopy. In addition to covering established methods and instrumentation, the contributors also identify potential new applications in a number of areas. Throughout, they emphasize the multifunctional and multidimensional uses of the marriage of fluorescence imaging spectroscopy and digital imaging microscopy. They show how these technologies can be used in combination to obtain distinct yet complementary information, allowing two- and three-dimensional measurements, as well as manipulations of the sample under study in real time. This book makes the field of fluorescence imaging spectroscopy and microscopy accessible to scientists in a variety of research fields. It is also an excellent text for graduate students, a reference to the latest techniques, and a source of ideas to stimulate future investigation. An exploration of ideas, methods, and applications in the exciting new area of fluorescence imaging spectroscopy and microscopy This book covers the latest in techniques and advances that have developed from the combined use of fluorescence imaging spectroscopy and video microscopy. A relatively new innovation, it has revolutionized molecular research in a wide range of disciplines. This approach allows acquisition of quantitative data regarding real-world biological processes--providing unprecedented insight into the physiology of living cells and their molecular structures and functions. Written by a diverse group of leading researchers who contribute from their respective scientific disciplines--and from their perspective at the cutting edge of the technology--this book * Combines a unified treatment and a critical evaluation of the field, presenting new ideas, methods, and instrumentation, with tips for developing new applications * Covers many new techniques in different scientific disciplines--from biology and chemistry to physics, engineering, and medicine * Emphasizes the multifunctional and the multidimensional uses of fluorescence spectroscopy and video microscopy, and shows how they can be applied in different combinations to obtain new information For biochemists, cell biologists, biophysicists, physiologists, neuroscientists, applied physicists, and materials scientists, as well as for scientists working in industrial, agricultural, medical, and pharmaceutical research--and for graduate students in these areas--this book is a novel resource of research opportunities, new ideas, and current practices in a dynamic and burgeoning new field.

Fluorescence Microscopy

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Author :
Release : 2020-08-26
Genre : Science
Kind : eBook
Book Rating : 505/5 ( reviews)

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Book Synopsis Fluorescence Microscopy by : B. Herman

Download or read book Fluorescence Microscopy written by B. Herman. This book was released on 2020-08-26. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.

Fluorescence Microscopy of Living Cells in Culture, Part B

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Author :
Release : 1989-04-01
Genre : Science
Kind : eBook
Book Rating : 283/5 ( reviews)

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Book Synopsis Fluorescence Microscopy of Living Cells in Culture, Part B by :

Download or read book Fluorescence Microscopy of Living Cells in Culture, Part B written by . This book was released on 1989-04-01. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence Microscopy of Living Cells in Culture, Part B

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