Author : Pranjal Singh
Release : 2021
Genre :
Kind : eBook
Book Rating : /5 ( reviews)
Book Synopsis A Coarse-Grained Molecular Dynamics Model of the Mammalian Nuclear Envelope by : Pranjal Singh
Download or read book A Coarse-Grained Molecular Dynamics Model of the Mammalian Nuclear Envelope written by Pranjal Singh. This book was released on 2021. Available in PDF, EPUB and Kindle. Book excerpt: The cell nucleus houses most of the genetic material in eukaryotic cells and is the site of many fundamental genome regulatory processes including cell division, gene expression, differentiation, and aging. The regulation of architecture of the nuclear envelope (NE) is essential for smooth operation of these processes. There is currently no physics-based model, theoretical or computational, which accounts for all significant interactions of subnuclear components in a common framework, leaving much of NE mechanics unexplored and poorly understood. We present a coarse-grained molecular dynamics model of the mammalian NE of unprecedented detail. A three-step protocol is shown to generate a coarse-grained nuclear envelope (CGNE) with experimentally consistent structure. The CGNE features a monolayer model of the nuclear membranes and a dynamically-linked meshwork model of the underlying nuclear lamina. Peripheral chromatin, which supports the meshwork from underneath and also interlaces with it, is modeled as a chain of mesoscale domains. The methodologies for in silico mechanical testing of the CGNE and extraction of metrics of NE viscoelasticity from the simulation data are described. Parameters are being tuned towards achieving experimental consistency of these viscoelastic properties. A revised CGNE model is planned to be published elsewhere in the near future.
