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Use of Fourier Transform Infrared Spectroscopy for the Identification of Bacteria of Importance to the Food Industry

2007 Electronic dissertations
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Release : 2007
Genre : Electronic dissertations
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Book Synopsis Use of Fourier Transform Infrared Spectroscopy for the Identification of Bacteria of Importance to the Food Industry by : Sarah Pegram

Download or read book Use of Fourier Transform Infrared Spectroscopy for the Identification of Bacteria of Importance to the Food Industry written by Sarah Pegram. This book was released on 2007. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this work was to use Fourier Transform Infrared Spectroscopy to characterize and identify bacteria of particular significance to the food industry. FT-IR spectroscopy is a rapid technique that can be applied to all groups of bacteria. The two objectives were to determine a suitable sampling procedure to record a spectrum and to determine a suitable statistical technique to identify characteristic regions of the spectrum associated with the genus and, potentially, the species. Pure cultures of bacteria were grown in broth, suspended in saline and dried to produce a film on a halide salt crystal. These films were then used to produce FT-IR spectra. In total, 80 spectra were recorded from seven genera, seven species and four strains of bacteria. Some of the spectra were considered to be too low in intensity to be included in statistical analysis. Data points from three specific windows of the remaining spectra were used to determine spectral distances between spectra. These spectral distances were used to perform cluster analysis using Ward's method, the Complete Linkage method and the Centroid method. The statistical analysis created successful clusters for several of the species used but was inconclusive overall in being able to distinguish between spectra at the genus, species and strain level. This may be due to inconsistent growth of bacteria and insufficient manipulation of the data. This study has shown the potential for FT-IR spectroscopy to be used to identify bacteria with significance for food but further development is needed to reproduce the consistent results demonstrated in current literature.

Use of Fourier Transform Infrared Spectroscopy for the Classification and Identification of Bacteria of Importance to the Food Industry

2007
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Release : 2007
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Kind : eBook
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Book Synopsis Use of Fourier Transform Infrared Spectroscopy for the Classification and Identification of Bacteria of Importance to the Food Industry by : Sarah Pegram

Download or read book Use of Fourier Transform Infrared Spectroscopy for the Classification and Identification of Bacteria of Importance to the Food Industry written by Sarah Pegram. This book was released on 2007. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this work was to use Fourier Transform Infrared Spectroscopy to characterize and identify bacteria of particular significance to the food industry. FT-IR spectroscopy is a rapid technique that can be applied to all groups of bacteria. The two objectives were to determine a suitable sampling procedure to record a spectrum and to determine a suitable statistical technique to identify characteristic regions of the spectrum associated with the genus and, potentially, the species. Pure cultures of bacteria were grown in broth, suspended in saline and dried to produce a film on a halide salt crystal. These films were then used to produce FT-IR spectra. In total, 80 spectra were recorded from seven genera, seven species and four strains of bacteria. Some of the spectra were considered to be too low in intensity to be included in statistical analysis. Data points from three specific windows of the remaining spectra were used to determine spectral distances between spectra. These spectral distances were used to perform cluster analysis using Ward's method, the Complete Linkage method and the Centroid method. The statistical analysis created successful clusters for several of the species used but was inconclusive overall in being able to distinguish between spectra at the genus, species and strain level. This may be due to inconsistent growth of bacteria and insufficient manipulation of the data. This study has shown the potential for FT-IR spectroscopy to be used to identify bacteria with significance for food but further development is needed to reproduce the consistent results demonstrated in current literature.

Fourier Transform Infrared Spectroscopy in Food Microbiology

2012-06-02 Technology & Engineering
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Release : 2012-06-02
Genre : Technology & Engineering
Kind : eBook
Book Rating : 136/5 ( reviews)

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Book Synopsis Fourier Transform Infrared Spectroscopy in Food Microbiology by : Avelino Alvarez-Ordóñez

Download or read book Fourier Transform Infrared Spectroscopy in Food Microbiology written by Avelino Alvarez-Ordóñez. This book was released on 2012-06-02. Available in PDF, EPUB and Kindle. Book excerpt: Vibrational spectroscopy techniques, which have traditionally been used to provide non-destructive, rapid, and relevant information on microbial systematics, are useful for classification and identification. In conjunction with advanced chemometrics, infrared spectroscopy enables the biochemical signatures from microbiological structures to be extracted and analysed. In addition, a number of recent studies have shown that Fourier Transform Infrared (FT-IR) spectroscopy can help to understand the molecular basis of events, such as the adaptive tolerance responses expressed by bacteria when exposed to stress conditions in the environment, i.e. environments that cells confront in food and during food processing. The proposed Brief will discuss the published experimental techniques, data-processing algorithms, and approaches used in FT-IR spectroscopy to assist in the characterization and identification of microorganisms, to assess the mechanisms of bacterial inactivation by food processing technologies and antimicrobial compounds, to monitor the spore and membrane properties of foodborne pathogens in changing environments, to detect stress-injured microorganisms in food-related environments, to assess dynamic changes in bacterial populations, and to study bacterial tolerance responses.

Feasibility Study on the Application of Fourier Transform Infrared Spectroscopy for the Rapid Identification of Bacteria of Public Health Significance

1994 Bacteria
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Release : 1994
Genre : Bacteria
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Book Synopsis Feasibility Study on the Application of Fourier Transform Infrared Spectroscopy for the Rapid Identification of Bacteria of Public Health Significance by : Jin Tao

Download or read book Feasibility Study on the Application of Fourier Transform Infrared Spectroscopy for the Rapid Identification of Bacteria of Public Health Significance written by Jin Tao. This book was released on 1994. Available in PDF, EPUB and Kindle. Book excerpt: "The infrared spectra of 14 bacteria were recorded by Fourier Transform Infrared (FTIR) spectroscopy. The effects of changes in growth conditions, such as temperature, growth medium, and incubation time, sampling conditions on the reproducibility of the spectra were investigated. The spectra of bacteria suspended in saline solution, in D$ sb2$O-saline and on plate films were obtained and stored in separate spectral libraries. The application of library search routines for differentiation and identification of the 14 bacteria was investigated. The spectral regions used for the library search include 1750-1560 cm$ sp{-1}$ and 1480-960 cm$ sp{-1}$, which contain bands mainly associated with proteins (secondary structure), lipids, and polysaccharides. An index produced by the library search, which indicates how much an "unknown spectrum" matches a library spectrum, is suggested as a criterion for the differentiation and identification of bacteria. A correct identification of five unknown samples shows the feasibility of using spectral library searching routines for identification of bacteria down to the species level by FTIR spectroscopy. This technique is rapid, easier, and more efficient than conventional microbiological and biochemical methods. Quantitative analysis of a mixture of Escherichia coli and Staphylococcus aureus by the partial-lest-square (PLS) technique was also evaluated. The analytical error was about 10%." --

Advances in Biotechnology for Food Industry

2018-02-03 Technology & Engineering
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Release : 2018-02-03
Genre : Technology & Engineering
Kind : eBook
Book Rating : 959/5 ( reviews)

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Book Synopsis Advances in Biotechnology for Food Industry by : Alexandru Mihai Grumezescu

Download or read book Advances in Biotechnology for Food Industry written by Alexandru Mihai Grumezescu. This book was released on 2018-02-03. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biotechnology for Food Industry, Volume Fourteen in the Handbook of Food Bioengineering series, provides recent insight into how biotechnology impacts the global food industry and describes how food needs are diverse, requiring the development of innovative biotechnological processes to ensure efficient food production worldwide. Many approaches were developed over the last 10 years to allow faster, easier production of widely used foods, food components and therapeutic food ingredients. This volume shows how biotechnological processes increase production and quality of food products, including the development of anti-biofilm materials to decrease microbial colonization in bioreactors and food processing facilities. Presents basic to advanced technological applications in food biotechnology Includes various scientific techniques used to produce specific desired traits in plants, animals and microorganisms Provides scientific advances in food processing and their impact on the environment, human health and food safety Discusses the development of controlled co-cultivations for reproducible results in fermentation processes in food biotechnology

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