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Release : 1983
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Book Synopsis Structure Analysis of OmpC, One of the Major Proteins in the Outer Membrane of E. Coli, by High Resolution Electron Microscopy by :

Download or read book Structure Analysis of OmpC, One of the Major Proteins in the Outer Membrane of E. Coli, by High Resolution Electron Microscopy written by . This book was released on 1983. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation is concerned with the structure analysis of a pore-forming membrane protein, OmpC, which is one of the major proteins in the outer membrane of Escherichia coli. In order to obtain structural information it was necessary to develop a suitable technique for preparing two-dimensional crystalline arrays of this membrane protein in an unfixed, unstained and hydrated condition. Electron micrographs were recorded at exposures of less than 5 electrons/A2 in order to avoid severe radiation damage. The resulting images were crystallographically averaged, in order to overcome the statistical limitations associated with the low electron exposures. The resulting images, which extend to a resolution of approx. 13.5 A, lend themselves to a natural interpretation that is consistent with the mass density of protein, water and lipid, prior data from 2-D and 3-D structure studies of negatively stained specimens at approx. = 20 A resolution, and published spectroscopic data on the peptide chain secondary structure.