Author : Bouzakri Karim
Release : 2017
Genre :
Kind : eBook
Book Rating : /5 ( reviews)
GET EBOOK
Book Synopsis A New Component of Skeletal Muscle Secretome Impact on Beta Cells and Prevent TNF-a and Cytomix Effect on Beta-cells by : Bouzakri Karim
Download or read book A New Component of Skeletal Muscle Secretome Impact on Beta Cells and Prevent TNF-a and Cytomix Effect on Beta-cells written by Bouzakri Karim. This book was released on 2017. Available in PDF, EPUB and Kindle. Book excerpt: A new positive component of skeletal muscle secretome impact on beta cells and prevent TNF-alpha and cytomix effect on beta cells.Karim Bouzakri, Julien Cherfan, Harzo Hamdard, Carole Mura, Claude Peronet, and Michel Pinget.UMR DIATHEC, EA 7294, Centre Europu00e9en du2019Etude du Diabu00e8te, Universitu00e9 de Strasbourg (UdS), Boulevard Renu00e9 Leriche 67200 Strasbourg, FranceBackground and aims: Inter-organ crosstalk including possible communication between skeletal muscle and beta cells is an important component of normal physiology that may contribute towards the pathophysiology of type 2 diabetes. Indeed, skeletal muscle cells secrete different sets of soluble proteins (myokines) which could act positively or negatively (in health and disease respectively) on the function of distant tissues including pancreatic islets. We have observed that conditioned medium from skeletal muscle of healthy individuals has positive effects on beta cell function. A new myokine, was identified as a cytokine regulated by muscle fiber type. The major aim of this study was to explore the impact of our new myokine (Myo X) on primary sorted beta cells under control conditions and after exposure to TNF-u03b1 or insulin resistance condition medium from skeletal muscle cells.Materials and methods: All studies were performed using sorted rat primary beta cells and human islets exposed to Myo X for 24h. Glucose-stimulated insulin secretion (GSIS) and glucagon secretion was measured by radioimmunoassay and ELISA after 1h at 2.8 mmol/l and/or 1h at 16.7mmol/l glucose. Cell death and proliferation were measured by TUNEL assay and BrdU incorporation respectively. To study the potential beneficial/protective effects of our myokine, beta cells were exposed to it for 24h alone or with addition 20 ng/ml TNF-u03b1 alone or insulin resistance condition medium from skeletal muscle cells. Data are mean u00b1 SE for 5-8 independent experiments.Results: Myo X treatment of rat primary beta cells and human islets for 24h increased GSIS (control: 12.4 u00b1 3.2% content/h; 1u00b5g/ml Myo X: 15.08 u00b1 3.8%*; 10 u00b5g/ml Myo X: 14.1 u00b1 1.8%*; *p