1# Library to Find Best eBook for Read
Author : Stephen Thorpe
Release : 2012
Genre :
Kind : eBook
Book Rating : /5 ( reviews)
Book Synopsis The Effect of Mechanical Cues on the Chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells by : Stephen Thorpe
Download or read book The Effect of Mechanical Cues on the Chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells written by Stephen Thorpe. This book was released on 2012. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Kee Woei Ng
Release : 2014-02-11
Genre : Technology & Engineering
Kind : eBook
Book Rating : 258/5 ( reviews)
Book Synopsis A Manual For Differentiation Of Bone Marrow-derived Stem Cells To Specific Cell Types by : Kee Woei Ng
Download or read book A Manual For Differentiation Of Bone Marrow-derived Stem Cells To Specific Cell Types written by Kee Woei Ng. This book was released on 2014-02-11. Available in PDF, EPUB and Kindle. Book excerpt: This is the first experimental protocol book that covers the differentiation of bone marrow-derived stem cells (BMSCs) into specific cell types, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds.BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost any kind of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissues such as blood vessels, heart, liver and so on.For the differentiation of BMSCs, many methods have been introduced to adjust their microenvironment (chemical and physical cues), including chemical induction methods using large or small molecules and pellet culture; mechanical stimulation induction methods using cyclic mechano-transduction or ultrasonication; cytokine-released method using scaffolds; and so on.
Author : Courtney Eileen LeBlon
Release : 2013
Genre :
Kind : eBook
Book Rating : 038/5 ( reviews)
Book Synopsis The Effect of Mechanical Cues on in Vitro Aging and Differentiation of Human Mesenchymal Stem Cells by : Courtney Eileen LeBlon
Download or read book The Effect of Mechanical Cues on in Vitro Aging and Differentiation of Human Mesenchymal Stem Cells written by Courtney Eileen LeBlon. This book was released on 2013. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering is a field that aims to replace or repair damaged tissue through the use of stem cells, biomaterials, and biomolecules. Human mesenchymal stem cells are multipotent adult stem cells that can be autologously transplanted. This work describes the effect of mechanical cues on human mesenchymal stem cells. An analysis on the age-related stiffening of these cells, and its effect on osteogenic and myogenic differentiation, is presented. This study gives insight to those using stem cells in vitro for extended periods of time. The effect of mechanical loading on stem cell differentiation is examined. Tensile and compressive loading are used to induce myogenic and osteogenic differentiation, respectively, in the absence of chemical cues. This study demonstrates that loading alone can accelerate differentiation. A 3-D cell culture method for cardiomyocyte differentiation is also explored. Numerous cardiomyocyte markers were observed, signifying that this method may be superior to chemical induction methods. A biodegradation study of four porous polymers is also presented, as scaffold choice is of great importance in the area of tissue engineering. This research provides guidance to those using human mesenchymal stem cells for tissue engineering.
Author : Lorenzo Fassina
Release : 2024-05-28
Genre : Technology & Engineering
Kind : eBook
Book Rating : 586/5 ( reviews)
Book Synopsis Cells, Biomaterials, and Biophysical Stimuli for Bone, Cartilage, and Muscle Regeneration, volume II by : Lorenzo Fassina
Download or read book Cells, Biomaterials, and Biophysical Stimuli for Bone, Cartilage, and Muscle Regeneration, volume II written by Lorenzo Fassina. This book was released on 2024-05-28. Available in PDF, EPUB and Kindle. Book excerpt: This Research Topic is Volume II of the article collection, Cells, Biomaterials, and Biophysical Stimuli for Bone, Cartilage, and Muscle Regeneration Over the last few years, a variety of tissue engineering strategies have been developed to improve the regeneration of bone, cartilage, and skeletal muscle. Numerous studies have proven that physical factors (external mechanical forces, and biomaterials’ features), as well as biochemical factors, may induce cells to reprogram their functions and dynamically adapt to the cellular microenvironment conditions. The advances in understanding the role of biophysical cues in the stem cells microenvironment point out the importance of their application in biomedicine and biotechnology to drive and modulate cell behavior for therapeutic purposes. In this context, many efforts are dedicated to design different strategies to engineer the physical aspects of the natural cellular microenvironment. The development of these technologies may be useful for identifying and studying the physical factors and help to clarify their downstream mechanisms to control cell behavior. This Research Topic will promote an overview of recent advances and cutting-edge approaches based on primary cells, stem cells, extracellular vesicles (EVs), biomaterial scaffolds, bioreactors, biophysical stimuli (e.g., mechanical forces, electromagnetic waves), and biochemical cues. All research involving one or more of the aforementioned cells and methods is welcome to elucidate new basic-research findings (e.g., molecular insights, biochemical pathways toward regeneration) and possible new clinical strategies (e.g., bioreactors for cell factories). An interdisciplinary design (e.g., biology/biochemistry plus bioengineering) is very welcome.
Author : 許婷恩
Release : 2017-01-27
Genre :
Kind : eBook
Book Rating : 380/5 ( reviews)
Book Synopsis In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels by : 許婷恩
Download or read book In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels written by 許婷恩. This book was released on 2017-01-27. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels" by 許婷恩, Ting-yan, Hui, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels submitted by Hui Ting Yan for the Degree of Master of Philosophy at The University of Hong Kong in August, 2007 Articular cartilage, the load-bearing tissue of the joint, has limited potential for repair and regeneration. Given a lack of satisfactory surgical solutions, cartilage tissue engineering has been suggested as a promising approach for cartilage repair. The present study demonstrated the fabrication of cartilage-like tissue-engineered constructs by chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in collagen gels in vitro. Collagen-hMSC constructs were synthesized with 5 6 varying cell seeding densities (110 - 510 cells/ml) and initial collagen concentrations (0.5 - 3 mg/ml) to investigate the effects of the two parameters. The constructs were cultured in chondrogenic differentiation induction medium for 21 days and evaluated by histological, immunohistochemial, morphological, biochemical and biomechanical examinations. In addition, with inclusion of chondroitin sulfate (CS), collagen/CS-hMSC constructs were also fabricated and compared to their collagen-hMSC constructs counterparts. After 21 days of culture, chondrogenesis was evident in the collagen-hMSC constructs, as indicated by positive immunohistochemical staining for cartilage-specific extracellular matrix components (type II collagen and aggrecan). The meshwork of collagen fibers was remodeled into a highly ordered microstructure, characterized by thick and parallel collagen bundles. Higher cell seeding density and higher collagen concentration favored the chondrogenic differentiation of the cells, yielding an increased matrix production (glycosaminoglycans) and a higher mechanical strength (reduced elastic modulus) of the constructs. A biochemical analysis of matrix accumulation revealed no difference between the collagen/CS-hMSC and collagen-hMSC constructs. Further investigation showed that most of the CS added during fabrication was lost to the surrounding medium within the first 24 hours. To study the effects of inclusion of CS, further studies should be performed to develop a fabrication method which enables effective CS incorporation into the constructs. The current work presented a systematic study to understand the parameters in building a cartilage-like construct, and provided the groundwork for optimization of the biological and mechanical characteristics. The findings may contribute towards the development of tissue engineering solutions for cartilage injuries. DOI: 10.5353/th_b3955874 Subjects: Chondrogenesis Collagen Stem cells Mesenchyme Tissue engineering
