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Surface Plasmon Enhanced, Coupled and Controlled Fluorescence

2017-03-03 Science
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Release : 2017-03-03
Genre : Science
Kind : eBook
Book Rating : 897/5 ( reviews)

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Book Synopsis Surface Plasmon Enhanced, Coupled and Controlled Fluorescence by : Chris D. Geddes

Download or read book Surface Plasmon Enhanced, Coupled and Controlled Fluorescence written by Chris D. Geddes. This book was released on 2017-03-03. Available in PDF, EPUB and Kindle. Book excerpt: Explains the principles and current thinking behind plasmon enhanced Fluorescence Describes the current developments in Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Details methods used to understand solar energy conversion, detect and quantify DNA more quickly and accurately, and enhance the timeliness and accuracy of digital immunoassays Contains contributions by the world’s leading scientists in the area of fluorescence and plasmonics Describes detailed experimental procedures for developing both surfaces and nanoparticles for applications in metal-enhanced fluorescence

Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy

2005
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Release : 2005
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Book Synopsis Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy by : Andreas Heinz Nicol

Download or read book Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy written by Andreas Heinz Nicol. This book was released on 2005. Available in PDF, EPUB and Kindle. Book excerpt:

Metal-Enhanced Fluorescence

2010-06-22 Science
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Release : 2010-06-22
Genre : Science
Kind : eBook
Book Rating : 785/5 ( reviews)

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Book Synopsis Metal-Enhanced Fluorescence by : Chris D. Geddes

Download or read book Metal-Enhanced Fluorescence written by Chris D. Geddes. This book was released on 2010-06-22. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.

Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy

2005
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Release : 2005
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Book Synopsis Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy by :

Download or read book Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy written by . This book was released on 2005. Available in PDF, EPUB and Kindle. Book excerpt: Over the last three decades, sensors based on the phenomenon of surface plasmon resonance have proven particularly suitable for real time thin film characterization, gas detection, biomolecular interaction examination and to supplement electrochemical methods. Systems based on prism coupling have been combined with fluorescence detection under the name of surface plasmon fluorescence spectroscopy to increase sensitivity even further. Alternatively, metal gratings can be employed to match photons for plasmon resonance. The real time monitoring of binding reactions not yet been reported in the combination of fluorescence detection and grating coupling. Grating-based systems promise more competitive products, because of reduced operating costs, and offer benefits for device engineering. This thesis is comprised of a comprehensive study of the suitability of grating coupling for fluorescence based analyte detection. Fundamental properties of grating coupled surface plasmon fluorescence spectroscopy are described, as well as issues related to the commercial realization of the method. Several new experimental techniques are introduced and demonstrated in order to optimize performance in certain areas and improve upon capabilities in respect to prism-based systems. Holographically fabricated gratings are characterized by atomic force microscopy and optical methods, aided by simulations and profile parameters responsible for efficient coupling are analyzed. The directional emission of fluorophores immobilized on a grating surface is studied in detail, including the magnitude and geometry of the fluorescence emission pattern for different grating constants and polarizations. Additionally, the separation between the minimum of the reflected intensity and the maximum fluorescence excitation position is examined. One of the key requirements for the commercial feasibility of grating coupling is the cheap and faithful mass production of disposable samples from a given master grat.

Surface Plasmon Enhanced Fluorescence for Biological Imaging

2020
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Release : 2020
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Book Synopsis Surface Plasmon Enhanced Fluorescence for Biological Imaging by : Shengnan Huang (Ph.D.)

Download or read book Surface Plasmon Enhanced Fluorescence for Biological Imaging written by Shengnan Huang (Ph.D.). This book was released on 2020. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence imaging offers high spatio-temporal resolution, low radiation dosage exposure, and low cost among all the available imaging modalities, for example, magnetic resonance imaging, computerized tomography and positron emission tomography. Imaging probes of high emissivity and photostability are the key to achieving fluorescence imaging with high signal-to-background ratio (SBR). One promising approach to developing highly bright and stable imaging probes is through surface plasmon enhanced fluorescence. In the first part of the thesis, we develop a fluorescent probe with high site-specificity and emission efficiency by exploiting the targeting-specificity of M13 virus and co-assembling plasmonic nanoparticles and visible dye molecules on the viral capsid. Practical factors controlling fluorescence enhancement, such as nanoparticle size and dye-to-nanoparticle distance, are studied in this project. Lastly, the highly fluorescent probe is applied for in vitro staining of E. coli. The methodology in this work is amendable to developing a wide range of affinity-targeted fluorescent probes using biotemplates. Compared to visible and near infrared spectrum, short-wave infrared (SWIR, 900-1700 nm) spectrum promises high spatial resolution and deep tissue penetration for fluorescence imaging of biological system, owning to low tissue autofluorescence and suppressed tissue scattering at progressively longer wavelengths. In the second part of the thesis, a bright SWIR imaging probe consisting of small SWIR dyes and gold nanorods is developed for in vivo imaging. Fluorescence enhancement is optimized by tuning the dye density on the gold nanorod surface. The SWIR imaging probes are applied for in vivo imaging of ovarian cancer. The effect of targeting modality on intratumor distribution of the imaging probes is studied in two different orthotopic ovarian cancer models. Lastly, we demonstrate that the plasmon enhanced SWIR imaging probe has great potential for fluorescence imaging-guided surgery by showing its capability to detect submillimeter-sized tumors. Apart from enhancing the SWIR down-conversion emission above, surface plasmon enhanced SWIR up-conversion emission is another promising approach to achieving "autofluorescence-free" imaging with minimal tissue scattering. In the third part of the thesis, we use gold nanorods to enhance the up-conversion emission of small SWIR dyes. The mechanism of surface plasmon enhanced up-conversion emission is studied. The up-conversion fluorescence shows much higher SBR than down-conversion fluorescence in non-scatting biological solution and scatting medium. Lastly, we demonstrate in vivo imaging for the first-time using SWIR up-conversion fluorescence with exceptional image contrast.

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