Author : M. S. Lesney
Release : 1980
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Kind : eBook
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Book Synopsis Studies on the Mechanisms of Plant Virus Infection in Two Leguminous Protoplast Systems. I. Bean Pod Mottle Virus Infection of Suspension Culture Derived Soybean Protoplast. II. Infection of Bean Leaf Protoplast with Bean Pod Mottle and Cowpea Mosaic Viruses by : M. S. Lesney

Download or read book Studies on the Mechanisms of Plant Virus Infection in Two Leguminous Protoplast Systems. I. Bean Pod Mottle Virus Infection of Suspension Culture Derived Soybean Protoplast. II. Infection of Bean Leaf Protoplast with Bean Pod Mottle and Cowpea Mosaic Viruses written by M. S. Lesney. This book was released on 1980. Available in PDF, EPUB and Kindle. Book excerpt: Protoplasts derived from soybean (Glycine max L. cv. 'Harosoy 63') liquid suspension cultures were inoculated with Bean Pod Mottle Virus (BPMW) in order to ascertain the effects of various inoculum amendments on the virus infection system with the ultimate goal of elucidating possible infection mechanisms. Among the parameters examined were the effects of virus concentration, protoplast concentration, buffer (pH and concentration), poly-ornithine (PLO), calcium or magnesium chloride, and temperature on percentage infection obtained. Virus concentration effects showed a sigmoidal increase in infection with concentration increase. Protoplast concentration showed an inverse relationship to infection. Potassium phosphate buffer showed a strong optimum for infection at pH 5.6. This was used as partial evidence for the involvement of histidine in the infection process. Effects of buffer concentration were pH dependent. PLO was not required for BPMV infection, but was found to be stimulatory. A synergistic effect on increasing virus infection was demonstrated between PLO and CaCl2 at low virus concentrations. CaCl2 proved more stimulatory to BPMV infec tion than did MgCl2. Pre-incubation experiments for both buffer and the divalent cations showed that pre- incubation with the virus was nessary for most of the stimulatory effect to be obtained, but that PLO-induced infection was actually somewhat improved wheh pre-incubation was not allowed: BPMV was relatively temperature independent in the presence of inoculum amendments (buffer, PLO, CaCl2), but temperature dependent in their absence; infection then was better at higher temperatures. Protoplasts were isolated from bean (Phaseolus vulgaris L. cv. 'Pencil Pod Wax ') leaves and infected with both BPMV and cowpea mosaic virus (CPMV) in order to compare infection for the two viruses in the two different systems. The same PLO/calcium synergy was evident as in the soybean system. The optimum pH in the bean system for BPMV was 5.6. The primary difference observed between the two ystems was the ability of pH 6.0 buffer to eliminate the PLO/calcium synergy when present in the bean system, but not in the soybean system for both viruses. The similarities seen between the leaf and suspension culture protoplasts were suggestive of the latter being equally 'natural' as a system, as well as one which is more reliable. The kind and number of the complexities seen in the two virus/protoplast systems were advanced as circumstantial evidence for the possibility of receptor mediated endocytosis as the mode of infection by plant viruses in protoplasts-especially when considering such a theory as an alternative to the membrane 'wouding' hypothesis.