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Single-particle Cryo-electron Microscopy

2017-12-31 Electron microscopy
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Release : 2017-12-31
Genre : Electron microscopy
Kind : eBook
Book Rating : 857/5 ( reviews)

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Book Synopsis Single-particle Cryo-electron Microscopy by : Joachim Frank

Download or read book Single-particle Cryo-electron Microscopy written by Joachim Frank. This book was released on 2017-12-31. Available in PDF, EPUB and Kindle. Book excerpt: The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.

Single-particle Cryo-EM of Biological Macromolecules

2021-05-19 Science
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Release : 2021-05-19
Genre : Science
Kind : eBook
Book Rating : 374/5 ( reviews)

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Book Synopsis Single-particle Cryo-EM of Biological Macromolecules by : GLAESER

Download or read book Single-particle Cryo-EM of Biological Macromolecules written by GLAESER. This book was released on 2021-05-19. Available in PDF, EPUB and Kindle. Book excerpt: This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. It is especially well suited for larger complexes and molecular machines. This book, provides a comprehensive, accessible and authoritative introduction to the field. It covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data-collection, data analysis, and the final validation of results. Key features Written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM Provides a comprehensive, accessible and authoritative introduction to the field of high-resolution structure analysis by single-article cryo-EM Covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data-collection, data analysis, and the final validation of results Authors of individual sections of this book have been recruited from among the most authoritative leaders in each topic

Cryo-EM Part A: Sample Preparation and Data Collection

2010-09-30 Science
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Release : 2010-09-30
Genre : Science
Kind : eBook
Book Rating : 955/5 ( reviews)

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Book Synopsis Cryo-EM Part A: Sample Preparation and Data Collection by :

Download or read book Cryo-EM Part A: Sample Preparation and Data Collection written by . This book was released on 2010-09-30. Available in PDF, EPUB and Kindle. Book excerpt: Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. Includes time-tested core methods and new innovations applicable to any researcher Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide

Biomolecular and Bioanalytical Techniques

2019-06-10 Science
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Release : 2019-06-10
Genre : Science
Kind : eBook
Book Rating : 964/5 ( reviews)

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Book Synopsis Biomolecular and Bioanalytical Techniques by : Vasudevan Ramesh

Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh. This book was released on 2019-06-10. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

Cryo-EM Part B: 3-D Reconstruction

2010-09-30 Science
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Release : 2010-09-30
Genre : Science
Kind : eBook
Book Rating : 926/5 ( reviews)

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Book Synopsis Cryo-EM Part B: 3-D Reconstruction by :

Download or read book Cryo-EM Part B: 3-D Reconstruction written by . This book was released on 2010-09-30. Available in PDF, EPUB and Kindle. Book excerpt: This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. Includes time-tested core methods and new innovations applicable to any researcher Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide

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