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Nanoscopy and Multidimensional Optical Fluorescence Microscopy

2010-04-26 Science
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Release : 2010-04-26
Genre : Science
Kind : eBook
Book Rating : 895/5 ( reviews)

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Book Synopsis Nanoscopy and Multidimensional Optical Fluorescence Microscopy by : Alberto Diaspro

Download or read book Nanoscopy and Multidimensional Optical Fluorescence Microscopy written by Alberto Diaspro. This book was released on 2010-04-26. Available in PDF, EPUB and Kindle. Book excerpt: "Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens

Far-Field Optical Nanoscopy

2015-02-07 Science
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Release : 2015-02-07
Genre : Science
Kind : eBook
Book Rating : 471/5 ( reviews)

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Book Synopsis Far-Field Optical Nanoscopy by : Philip Tinnefeld

Download or read book Far-Field Optical Nanoscopy written by Philip Tinnefeld. This book was released on 2015-02-07. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Optical Nanoscopy and Novel Microscopy Techniques

2014-09-19 Science
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Release : 2014-09-19
Genre : Science
Kind : eBook
Book Rating : 29X/5 ( reviews)

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Book Synopsis Optical Nanoscopy and Novel Microscopy Techniques by : Peng Xi

Download or read book Optical Nanoscopy and Novel Microscopy Techniques written by Peng Xi. This book was released on 2014-09-19. Available in PDF, EPUB and Kindle. Book excerpt: Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades. The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research. The book begins with coverage of super-resolution optical microscopy, which discusses targeted modulation such as STED and SIM or localization methods such as PALM. It then discusses novel development of fluorescent probes, such as organic small-molecule probes, fluorescent proteins, and inorganic labels such as quantum dots. Finally, it describes advanced optical microscopy, such as fluorescence lifetime imaging, fiber optic microscopy, scanning ion conductance microscopy, and the joining of optics and acoustics—photoacoustic microscopy. Following each chapter, a detailed list of references is provided. Problems at the end of each chapter are also included.

Optical Fluorescence Microscopy

2010-11-25 Science
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Release : 2010-11-25
Genre : Science
Kind : eBook
Book Rating : 752/5 ( reviews)

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Book Synopsis Optical Fluorescence Microscopy by : Alberto Diaspro

Download or read book Optical Fluorescence Microscopy written by Alberto Diaspro. This book was released on 2010-11-25. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Focus on Multidimensional Microscopy

1999 Science
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Release : 1999
Genre : Science
Kind : eBook
Book Rating : 920/5 ( reviews)

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Book Synopsis Focus on Multidimensional Microscopy by : Ping-chin Cheng

Download or read book Focus on Multidimensional Microscopy written by Ping-chin Cheng. This book was released on 1999. Available in PDF, EPUB and Kindle. Book excerpt: This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.

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