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Molecular Biology and Genetic Engineering of Yeasts

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Release : 2018-01-18
Genre : Technology & Engineering
Kind : eBook
Book Rating : 638/5 ( reviews)

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Book Synopsis Molecular Biology and Genetic Engineering of Yeasts by : Henri Heslot

Download or read book Molecular Biology and Genetic Engineering of Yeasts written by Henri Heslot. This book was released on 2018-01-18. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.

Yeast Metabolic Engineering

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Release : 2022-07-04
Genre : Science
Kind : eBook
Book Rating : 990/5 ( reviews)

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Book Synopsis Yeast Metabolic Engineering by : Valeria Mapelli

Download or read book Yeast Metabolic Engineering written by Valeria Mapelli. This book was released on 2022-07-04. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Yeast Genetics

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Release : 2012-12-06
Genre : Science
Kind : eBook
Book Rating : 914/5 ( reviews)

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Book Synopsis Yeast Genetics by : J.F.T. Spencer

Download or read book Yeast Genetics written by J.F.T. Spencer. This book was released on 2012-12-06. Available in PDF, EPUB and Kindle. Book excerpt: During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. There great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspectiv(}-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.

Guide to Yeast Genetics and Molecular Biology

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Release : 2004-05-14
Genre : Science
Kind : eBook
Book Rating : 137/5 ( reviews)

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Book Synopsis Guide to Yeast Genetics and Molecular Biology by :

Download or read book Guide to Yeast Genetics and Molecular Biology written by . This book was released on 2004-05-14. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein

Yeast Genetics

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Release : 2016-09-17
Genre : Science
Kind : eBook
Book Rating : 826/5 ( reviews)

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Book Synopsis Yeast Genetics by : Jeffrey S. Smith

Download or read book Yeast Genetics written by Jeffrey S. Smith. This book was released on 2016-09-17. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

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