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Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition

2003-11-24 Science
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Release : 2003-11-24
Genre : Science
Kind : eBook
Book Rating : 154/5 ( reviews)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition by : Leland J. Cseke

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition written by Leland J. Cseke. This book was released on 2003-11-24. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Handbook of Molecular and Cellular Methods in Biology and Medicine

2004 Cytology
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Release : 2004
Genre : Cytology
Kind : eBook
Book Rating : 561/5 ( reviews)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by :

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by . This book was released on 2004. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition

2011-12-12 Science
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Release : 2011-12-12
Genre : Science
Kind : eBook
Book Rating : 381/5 ( reviews)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition by : Leland J. Cseke

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition written by Leland J. Cseke. This book was released on 2011-12-12. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

Handbook of Molecular and Cellular Methods in Biology and Medicine

2004-11-23 Science
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Release : 2004-11-23
Genre : Science
Kind : eBook
Book Rating : 243/5 ( reviews)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Donghern Kim

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Donghern Kim. This book was released on 2004-11-23. Available in PDF, EPUB and Kindle. Book excerpt: Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Handbook of Molecular and Cellular Methods in Biology and Medicine

1995-05-15 Science
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Release : 1995-05-15
Genre : Science
Kind : eBook
Book Rating : 113/5 ( reviews)

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Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Donghern Kim

Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Donghern Kim. This book was released on 1995-05-15. Available in PDF, EPUB and Kindle. Book excerpt: Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

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