Gel Chromatography

2013-06-29 Science

Author : Helmut Determann
Release : 2013-06-29
Genre : Science
Kind : eBook
Book Rating : 064/5 ( reviews)

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Book Synopsis Gel Chromatography by : Helmut Determann

Download or read book Gel Chromatography written by Helmut Determann. This book was released on 2013-06-29. Available in PDF, EPUB and Kindle. Book excerpt: The efforts spent on many a scientific book cannot be justified, no matter how many words are said about it. The opposite is true for this book and a few brief remarks upon its publication. Within a short period of time, short even by all present standards, gel chromatography has gone through a development and experienced an acceptance that is unknown to any other method. From experience, the new and unique separation technique is today known and liked in all laboratories that are concerned with substances of high molecular weight; in others, the technique is known from hearsay, the least. Soon it became evident that a comprehensive coverage of the conceptual development, the theoretical principles, and the experimental technique of the new method would be desirable. This coverage is now offered by the book of an expert. Its author has personally participated in the development from its beginning and helped to promote it. He has made possible the gel chromatography, also of proteins, on thin layer plates; for lipophilic substances he has contributed considerably to the transition from water to organic solvent systems and developed theoretical concepts for a better understanding of the effects that are responsible for the separation. The book, so it appears to me, is pointing in new directions. The reader does not only expect a clear presentation of facts but also that of instructions for practical applications. Both these expectations have been met by the expert.

Protein Chromatography

2011-04-06 Science

Author : Dermot Walls
Release : 2011-04-06
Genre : Science
Kind : eBook
Book Rating : 147/5 ( reviews)

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Book Synopsis Protein Chromatography by : Dermot Walls

Download or read book Protein Chromatography written by Dermot Walls. This book was released on 2011-04-06. Available in PDF, EPUB and Kindle. Book excerpt: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Modern Size-Exclusion Liquid Chromatography

2009-07-31 Science

Author : Andre Striegel
Release : 2009-07-31
Genre : Science
Kind : eBook
Book Rating : 832/5 ( reviews)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : Andre Striegel

Download or read book Modern Size-Exclusion Liquid Chromatography written by Andre Striegel. This book was released on 2009-07-31. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Modern Size-Exclusion Liquid Chromatography

1979-09-27 Science

Author : W. W. Yau
Release : 1979-09-27
Genre : Science
Kind : eBook
Book Rating : /5 ( reviews)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : W. W. Yau

Download or read book Modern Size-Exclusion Liquid Chromatography written by W. W. Yau. This book was released on 1979-09-27. Available in PDF, EPUB and Kindle. Book excerpt: A critical treatment of all aspects, containing definitive examples, detailed description of data handling, compilations of information on columns and column packings, and tables of important solvents and standard reference materials. Combines a basic, unified theory with practical know-how, integrating methods of gel permeation and gel filtration chromatography. Provides an understanding of molecular characterization problems as well as a background for developing new and improved characterization methods and apparatus for HPSEC performed with columns of small particles at high pressures.

Column Handbook for Size Exclusion Chromatography

1999-03-19 Science

Author : Chi-san Wu
Release : 1999-03-19
Genre : Science
Kind : eBook
Book Rating : 399/5 ( reviews)

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Book Synopsis Column Handbook for Size Exclusion Chromatography by : Chi-san Wu

Download or read book Column Handbook for Size Exclusion Chromatography written by Chi-san Wu. This book was released on 1999-03-19. Available in PDF, EPUB and Kindle. Book excerpt: Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results

Popular eBooks

Gel Chromatography

Protein Chromatography

Modern Size-Exclusion Liquid Chromatography

Modern Size-Exclusion Liquid Chromatography

Column Handbook for Size Exclusion Chromatography

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