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FRET - Förster Resonance Energy Transfer

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Release : 2013-10-17
Genre : Science
Kind : eBook
Book Rating : 049/5 ( reviews)

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Book Synopsis FRET - Förster Resonance Energy Transfer by : Igor L. Medintz

Download or read book FRET - Förster Resonance Energy Transfer written by Igor L. Medintz. This book was released on 2013-10-17. Available in PDF, EPUB and Kindle. Book excerpt: FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.

FRET and FLIM Techniques

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Release : 2011-07-29
Genre : Science
Kind : eBook
Book Rating : 124/5 ( reviews)

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Book Synopsis FRET and FLIM Techniques by : Theodorus W. J. Gadella

Download or read book FRET and FLIM Techniques written by Theodorus W. J. Gadella. This book was released on 2011-07-29. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Fluorescence Microscopy

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Release : 2017-03-27
Genre : Medical
Kind : eBook
Book Rating : 726/5 ( reviews)

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Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck

Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck. This book was released on 2017-03-27. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Applied Nanophotonics

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Release : 2018-11-22
Genre : Science
Kind : eBook
Book Rating : 503/5 ( reviews)

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Book Synopsis Applied Nanophotonics by : Hilmi Volkan Demir

Download or read book Applied Nanophotonics written by Hilmi Volkan Demir. This book was released on 2018-11-22. Available in PDF, EPUB and Kindle. Book excerpt: An accessible yet rigorous introduction to nanophotonics, covering basic principles, technology, and applications in lighting, lasers, and photovoltaics. Providing a wealth of information on materials and devices, and over 150 color figures, it is the 'go-to' guide for students in electrical engineering taking courses in nanophotonics.

Principles of Fluorescence Spectroscopy

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Release : 2013-11-11
Genre : Science
Kind : eBook
Book Rating : 58X/5 ( reviews)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz. This book was released on 2013-11-11. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

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