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Biophysical Characterization of Functional Peptides

2023-08-02 Science
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Release : 2023-08-02
Genre : Science
Kind : eBook
Book Rating : 054/5 ( reviews)

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Book Synopsis Biophysical Characterization of Functional Peptides by : Vibin Ramakrishnan

Download or read book Biophysical Characterization of Functional Peptides written by Vibin Ramakrishnan. This book was released on 2023-08-02. Available in PDF, EPUB and Kindle. Book excerpt: This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial. ​

Biophysical Characterisation of Peptides and Proteins

2010
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Release : 2010
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Book Synopsis Biophysical Characterisation of Peptides and Proteins by : Angeliki Damianoglou

Download or read book Biophysical Characterisation of Peptides and Proteins written by Angeliki Damianoglou. This book was released on 2010. Available in PDF, EPUB and Kindle. Book excerpt:

Characterization of Proteins

2007-10-03 Science
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Release : 2007-10-03
Genre : Science
Kind : eBook
Book Rating : 379/5 ( reviews)

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Book Synopsis Characterization of Proteins by : Felix Franks

Download or read book Characterization of Proteins written by Felix Franks. This book was released on 2007-10-03. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell’s genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as “industrial components,” e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized.

Mass Spectrometry of Proteins and Peptides

2008-02-05 Science
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Release : 2008-02-05
Genre : Science
Kind : eBook
Book Rating : 454/5 ( reviews)

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Book Synopsis Mass Spectrometry of Proteins and Peptides by : John R. Chapman

Download or read book Mass Spectrometry of Proteins and Peptides written by John R. Chapman. This book was released on 2008-02-05. Available in PDF, EPUB and Kindle. Book excerpt: Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.

The Peptides Analysis, Synthesis, Biology

2012-12-02 Science
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Release : 2012-12-02
Genre : Science
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Book Rating : 634/5 ( reviews)

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Book Synopsis The Peptides Analysis, Synthesis, Biology by : Erhard Gross

Download or read book The Peptides Analysis, Synthesis, Biology written by Erhard Gross. This book was released on 2012-12-02. Available in PDF, EPUB and Kindle. Book excerpt: The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.

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