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Author : F. Sierra
Release : 2013-11-21
Genre : Science
Kind : eBook
Book Rating : 837/5 ( reviews)
Book Synopsis A Laboratory Guide to In Vitro Transcription by : F. Sierra
Download or read book A Laboratory Guide to In Vitro Transcription written by F. Sierra. This book was released on 2013-11-21. Available in PDF, EPUB and Kindle. Book excerpt:
Author : F. Sierra
Release : 1990-08-01
Genre : Science
Kind : eBook
Book Rating : 578/5 ( reviews)
Book Synopsis A Laboratory Guide to In Vitro Transcription by : F. Sierra
Download or read book A Laboratory Guide to In Vitro Transcription written by F. Sierra. This book was released on 1990-08-01. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert E. Farrell Jr.
Release : 2010-07-22
Genre : Science
Kind : eBook
Book Rating : 763/5 ( reviews)
Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.
Download or read book RNA Methodologies written by Robert E. Farrell Jr.. This book was released on 2010-07-22. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author : Jost
Release : 2013-03-13
Genre : Science
Kind : eBook
Book Rating : 614/5 ( reviews)
Book Synopsis A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions by : Jost
Download or read book A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions written by Jost. This book was released on 2013-03-13. Available in PDF, EPUB and Kindle. Book excerpt: A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.
Author : Martin J. Tymms
Release : 2008-02-02
Genre : Science
Kind : eBook
Book Rating : 243/5 ( reviews)
Book Synopsis In Vitro Transcription and Translation Protocols by : Martin J. Tymms
Download or read book In Vitro Transcription and Translation Protocols written by Martin J. Tymms. This book was released on 2008-02-02. Available in PDF, EPUB and Kindle. Book excerpt: Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.
