Author : Leon D. Betowski
Release : 1989
Genre : High performance liquid chromatography
Kind : eBook
Book Rating : /5 ( reviews)
Book Synopsis Application of High Performance Liquid Chromatography/mass Spectrometry to Environmental Analysis by : Leon D. Betowski
Download or read book Application of High Performance Liquid Chromatography/mass Spectrometry to Environmental Analysis written by Leon D. Betowski. This book was released on 1989. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) is described for the analysis of samples for environmental pollutants. During the course of this work different techniques were used to increase both the sensitivity and specificity of HPLC/MS. These included the utilization of two different interfaces for the mass spectrometer - the thermospray and the particle beam systems. Refinements to the thermospray system were made with the use of a wire-repeller to enhance sensitivity and also to increase the structural information obtained from mass spectra. The operation of the instrument in the discharge negative ionization mode further increased the specificity of these techniques as did the use of a triple quadrupole mass spectrometer in the HPLC/MS system. Two different sets of compounds were used in this study. Organic dyes were used to test the improvements in the system. Since work was performed with dyes previously and since dye compounds encompass a variety of structural functionalities, the dyes made good test compounds. Chlorinated herbicides were studied in an effort to analyze for these compounds directly without having to prepare derivatives before separation.